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KMID : 0613820110210070976
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Journal of Life Science
2011 Volume.21 No. 7 p.976 ~ p.984
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Application of Isolated Tyrosinase Inhibitory Compounds from Persimmon Leaves
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Cho Young-Je
An Bong-Jeun
Kim Jung-Hwan
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Abstract
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Total phenolic content was the highest in 60% ethanol extracts at 21.91 ㎎/g, and inhibitory activity against tyrosinase of 60% ethanol extracts was higher than ethanol extracts of other concentration. The inhibitory compounds against tyrosinase from Persimmon leaves were purified using Sephadex LH-20, MCI-gel CHP-20 column chromatography with gradient elution. Two purified compounds were isolated as a result. The chemical structures of each compound were determined and identified using ¹H-NMR and ¹³C-NMR, FAB-Mass. The compounds were confirmed as (+)-gallocatechin and prodelphinidin B-3. The tyrosinase inhibitory activities of purified (+)-gallocatechin and prodelphinidin B-3 were 29.5 and 40.2%, respectively. The inhibitory activities of (+)-gallocatechin and prodelphinidin B-3 against melanin biosynthesis in melanoma cell were 32.5 and 46.7%. The safety of essence with tyrosinase inhibitory compounds from persimmon leaves was also assessed by various safety profiles. First, changes in pH (4.90∼4.95) and viscosity (23,000∼26,000 cP) was not detected for 60 days. Essence also showed stability against temperature and light for 60 days. All these findings suggest that extracts from persimmon leaves have a great potential as a cosmetical ingredient with a potent whitening effect.
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KEYWORD
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Application, tyrosinase inhibition, phenolics, persimmon leaves
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